construction and est sequencing of bmc plant biology

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Construction and EST sequencing of ... - BMC Plant Biology

2011-11-25  Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.) Matthew W Blair 1, Andrea C Fernandez 1, Manabu Ishitani 1, Danilo Moreta 1, Motoaki Seki 2, Sarah Ayling 1 Kazuo Shinozaki 2 BMC Plant Biology volume 11, Article number: 171 (2011) Cite this article

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Construction and EST sequencing of ... - BMC Plant Biology

2017-8-25  Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.) Blair et al. Blair et al. BMC Plant Biology 2011, 11:171

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(PDF) Construction and EST sequencing of full-length ...

2011-11-25  Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.) November 2011 BMC Plant Biology 11(1):171

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Construction and EST sequencing of full-length, drought ...

2011-11-25  In comparisons of the full-length cDNA library assembly with EST sequencing efforts of Ramírez et al. and Thibivilliers et al. we found that about half of the sequences were novel and a significant number represented the 5' portions of previously discovered genes from other EST efforts as shown by the diagrams in Figure Figure2 2 and and3. 3.

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Construction of 12 EST libraries and ... - BMC Plant Biology

2009-1-28  Sequencing and clustering. All cDNA libraries were non-normalized. Ten root libraries were generated from a single chicory cultivar (Arancha) harvested during growing season 2002 (figure 1), providing a season-wide overview of the genes expressed in chicory roots.The first letter (R-root, F-leaf, N-nodule) refers to the organ from which RNA were extracted while the second letter refers to the ...

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BMC Plant Biology BioMed Central - CORE

2015-6-9  Sequencing EST clones from such libraries is a first step toward isolating and identifying gene sequences involved in the plant-nematode interaction. The collection of EST sequence information requires accu-rate gene annotation as well as dedicated platforms for storage, processing, curation and data retrieval. Ideally,

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GarlicESTdb: an online database and ... - BMC Plant Biology

2009-5-18  EST sequencing is a useful tool for investigating a wide variety of genetic characteristics of a species, such as how many genes exist in the species, how gene expression patterns differ between tissues, where exonic and intronic regions are located and how many alternatively spliced transcripts can be created from a single gene [1–3].In addition, ESTs have proven to be extremely useful for ...

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BMC Plant Biology Home page

BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.

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Construction of a high-density genetic ... - BMC Plant

2019-5-30  Some broccoli (Brassica oleracea L. italic) accessions have purple sepals and cold weather would deepen the purple color, while the sepals of other broccoli lines are always green even in cold winter. The related locus or gene is still unknown. In this study, a high-density genetic map was constructed based on specific locus amplified fragment (SLAF) sequencing in a doubled-haploid

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Construction and analysis of a high-density ... - BMC

2012-10-3  Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a

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BMC Plant Biology BioMed Central - CORE

2015-6-9  Sequencing EST clones from such libraries is a first step toward isolating and identifying gene sequences involved in the plant-nematode interaction. The collection of EST sequence information requires accu-rate gene annotation as well as dedicated platforms for storage, processing, curation and data retrieval. Ideally,

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BMC Plant Biology BioMed Central - Springer

2017-8-27  BMC Plant Biology BMC Plant Biology 2002, 2Methodology article x ... sequencing and clustering of 57,000 EST sequences yield-ed 12,000 non-overlapping sequence clusters, and ... construction of a cDNA library transformation of Physcomitrella with the gene disruption cDNA library

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BMC Plant Biology BioMed Central - link.springer

2017-8-24  BMC Plant Biology Research article Open Access Generation and analysis of 9792 EST sequences from cold acclimated oat, Avena sativa Marcus Bräutigam1, Angelica Lindlöf2, Shakhira Zakhrabekova1, Gokarna Gharti-Chhetri 1, Björn Olsson*2 and Olof Olsson*

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BMC Plant Biology BioMed Central

2021-7-9  BMC Plant Biology Research article Open Access ... We have searched homologs for 118 Arabidopsis flowering time genes from Fragaria by EST sequencing and bioinformatics analysis and identified 66 gene homologs that by sequence similarity, ... Construction and sequencing

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Construction and analysis of a high-density ... - BMC

2012-10-3  Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a

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Genomic resources for Myzus persicae: EST sequencing,

Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design BMC Genomics . 2007 Nov 16;8:423. doi: 10.1186/1471-2164-8-423.

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Construction of a highly saturated Genetic Map for Vitis ...

Background: High-saturate molecular linkage maps are an important tool in studies on plant molecular biology and assisted breeding. Development of a large set of single nucleotide polymorphisms (SNPs) via next-generation sequencing (NGS)-based methods, restriction-site associated DNA sequencing (RAD-seq), and the generation of a highly saturated genetic map help improve fine mapping of ...

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Construction of a high-density genetic map and mapping

Background: Undaria pinnatifida is an important economic brown alga in East Asian countries. However, its genetic and genomic information is very scarce, which hinders further research in this species. A high-density genetic map is a basic tool for fundamental and applied research such as discovery of functional genes and mapping of quantitative trait loci (QTL).

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Construction of a high-density genetic map by specific ...

Background: Upland Cotton (Gossypium hirsutum) is one of the most important worldwide crops it provides natural high-quality fiber for the industrial production and everyday use. Next-generation sequencing is a powerful method to identify single nucleotide polymorphism markers on a large scale for the construction of a high-density genetic map for quantitative trait loci mapping.

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Plant Methods Home page

2021-8-11  Aims and scope Plant Methods is an open access, peer-reviewed journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.. The goal of this journal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration

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BMC Plant Biology - hal.archives-ouvertes

2021-7-9  EST-SSR and 60 SNP -based markers identified from oak transcriptome sequence information (31,798 Sanger -based unigenes from Ueno et al. [33]). These four objectives are interconnected, as shown in Figure 1.

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Construction and analysis of a high-density ... - BMC

2012-10-3  Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a

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EST sequencing and SSR marker development from

EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.) ... and library construction followed the protocol of Stratagene's pBluescript II cDNA library construction kit. Sequencing and ESTs analysis. Plasmid DNA was isolated from the randomly selected colonies and preserved in 96-well plate. ... BMC Plant Biology ...

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BMC Plant Biology BioMed Central

2021-7-9  BMC Plant Biology Research article Open Access ... We have searched homologs for 118 Arabidopsis flowering time genes from Fragaria by EST sequencing and bioinformatics analysis and identified 66 gene homologs that by sequence similarity, ... Construction and sequencing

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(PDF) The first set of EST resource for gene discovery and ...

Figure 11 A snapsh ot of sequence alignment of EST seque nces and amplicons for cont ig 433 validating the in silico predicted SNP. ... BMC Plant Biology 2010, 10:45.

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Construction of a plant-transformation ... - BMC Genomics

2013-3-28  Cotton, one of the world’s leading crops, is important to the world’s textile and energy industries, and is a model species for studies of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. Here, we report the construction of a plant-transformation-competent binary bacterial artificial chromosome (BIBAC) library and comparative genome sequence analysis of polyploid ...

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ASIP Home - PlantGDB - Resources for Plant Comparative ...

ASIP Publication: BMC plant biology 2008 - Cross-Species EST Alignments Reveal Novel and Conserved Alternative Splicing Events in Legumes ASIP updated @ 6/30/2007; Legumes come to ASIP, thanks to the Medicago Genome Sequencing Project.. Medicago and Lotus data identified from same-species and cross-species EST/cDNAs.

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Plant Methods Home page

2021-8-11  Aims and scope Plant Methods is an open access, peer-reviewed journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.. The goal of this journal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration

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Construction of a high-density genetic map by specific ...

Background: Upland Cotton (Gossypium hirsutum) is one of the most important worldwide crops it provides natural high-quality fiber for the industrial production and everyday use. Next-generation sequencing is a powerful method to identify single nucleotide polymorphism markers on a large scale for the construction of a high-density genetic map for quantitative trait loci mapping.

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Optimized sequencing depth and de novo assembler for ...

Optimized sequencing depth and de novo assembler for deeply reconstructing the transcriptome of the tea plant, an economically important plant species BMC Bioinformatics . 2019 Nov 6;20(1):553. doi: 10.1186/s12859-019-3166-x.

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